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Analytical Chemistry
Cool Science

A Step Closer To Roadside Cannabis Sensing

19 Apr 2020
- by Dr Alison Halliday

Scientific Cannabis

Researchers develop a simple new test that can determine if a person has used a THC–containing product by analysing the sweat content of their fingerprints.

Across the world, marijuana is growing in popularity. A reported 182.5 million people are users – nearly 4% of the global population. In the US, the recreational use of marijuana is legal in 11 states, a further 22 have legalised it for medicinal purposes, with varying degrees of decriminalisation in 15 others.

But despite these steps, there are still no regulations regarding the excessive use of cannabis products – and so there is a zero-tolerance policy when it comes to driving and operating machinery. Lawmakers face the challenge of establishing formal intoxication levels and developing reliable methods to measure them.

Tetrahydrocannabinol (THC) is the main psychoactive compound of marijuana. However, as this is quickly broken down within the body, researchers instead need to develop ways to detect its metabolites, including THC–COOH. While several analytical techniques have already been developed to detect these compounds in saliva, blood, urine, hair or nails, there still remains a greater need for the ability to conduct roadside testing.

A Simple, Effective Assay for Detecting THC Metabolites

In a new study, published in ACS Sensors, a team of researchers set out to develop a simple new test that can detect THC metabolites in fingerprint sweat.1The researchers designed a competitive immunoassay, which is based on the premise that anti–THC antibodies have a greater affinity for THC metabolites than THC.

In the system, a sweat sample containing THC, THC–COOH or other metabolites, would cause a decrease in the response of the assay that is easily measured using UV spectroscopy.

Fingerprint Sweat Testing

Following optimisation, the team tested the immunoassay on real fingerprints from a sober volunteer, which they spiked with varying concentrations of THC–COOH. They successfully demonstrated that it was possible to detect the metabolite at levels from 5.0 nM to 100 nM – without meaningful interference from other fingerprint sweat components.

The researchers then analysed fingerprints from three active marijuana users, showing that the absorbance of the assay decreased when an individual was actively high compared with the response observed in samples from non–users. 

For all experiments, the researchers used ultrapure water generated from an ELGA PURELAB® flex laboratory water purification system to minimise the risk of adding contaminants that may affect their results.

A Sensitive, Fast and Easy–to–Use Platform

In this study, the researchers developed a sensitive new method for detecting THC metabolites in the sweat content of their fingerprints, which could one day help law enforcement to determine whether someone has used a THC–containing product.

As this approach could be readily combined with a strip-like design, it could provide a simple, on–the–spot test that requires little or no scientific training.
With low detection capabilities and the ability to differentiate between a narrow range of concentrations, it also has the potential to measure the levels of THC and its metabolites in a person’s body – once formal intoxication levels are established.

Why Choose ELGA LabWater?

ELGA’s expert engineers, chemists and scientists are at the forefront of technological innovation. We continue to introduce game-changing features to the laboratory water market.

Reference:

  1. Brunelle, E. et al. Step toward Roadside Sensing: Noninvasive Detection of a THC Metabolite from the Sweat Content of Fingerprints. ACS Sens. 2019: 4(12): 3318–3324.


Dr Alison Halliday
After completing an undergraduate degree in Biochemistry & Genetics at Sheffield University, Alison was awarded a PhD in Human Molecular Genetics at the University of Newcastle. She carried out five years as a Senior Postdoctoral Research Fellow at UCL, investigating the genes involved in childhood obesity syndrome. Moving into science communications, she spent ten years at Cancer Research UK engaging the public about the charity’s work. She now specialises in writing about research across the life sciences, medicine and health.

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Highlights

18 Jan 2021
Forensic Toxicological Analysis Of Cannabinoids In Blood
14 Dec 2020
Developing Batteries Powered by Human Urine 
23 Nov 2020
Improving the Treatment of Diabetic Foot Infections
16 Nov 2020
Does The Type Of Wine Fermentation Vessel Matter?
9 Nov 2020
Investigating ‘DNA Vaccines’ to Prevent Hepatitis B Infection
12 Oct 2020
Printing Personalised Medical Cannabis Products
5 Oct 2020
Can Microscopic Needles Help Treat Skin Cancer?
21 Sep 2020
Turning Mushrooms into Versatile Materials

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