Let's talk about lab water
Let's talk about lab water
Grapevine diseases can cause a significant impact on fruit yield. These include downy mildew, which is caused by Plasmopara viticola and affects all green parts of the grapevine – and grey mould, which is caused by the fungal pathogen Botrytis cinerea and leads to fruit rot.
But plants have evolved natural defences – including producing chemicals called phytoalexins that act against attacking pathogens. For example, stilbenes are a group of polyphenols produced by grapevines in response to an infection. Many of these substances, especially those that form oligomeric structures, are known to have high antimicrobial activity.
Sourcing stilbenes from vine-growing by-products, such as grapevine canes and roots, is attracting growing interest due to their potential for fighting diseases like downy mildew and grey mould. But the low efficiency of natural grapevine extracts against common grape pathogens currently prevents their application in the field.
In a new study, published in OENO One, researchers explore using a chemical process to improve the effectiveness of natural grapevine–cane extract against downy mildew and grey mould.1
The researchers applied oxidative coupling using silver acetate directly on an enriched natural grapevine cane extract containing three main stilbenes (trans-resveratrol, trans– ϵ–viniferin and r–viniferin). Their subsequent analysis of the processed extract using ultra–high-performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC–DAD–MS) indicated the formation of at least eleven compounds.
After further purification, mass spectrometry and NMR spectroscopy analysis, the team went on to isolate and identify the structure of a total of 10 compounds. These included four newly reported compounds: trans–oxistilbenin C, trans–oxistilbenin D and cis– and trans–oxistilbenin E.
The researchers then compared the antimicrobial activity of the initial grapevine–cane extract with the product obtained after oxidative coupling.
These experiments showed that their chemically engineered extract was three times more active than the original extract against downy mildew – and was also more effective at preventing the growth of B. cinerea.
The team used analytical grade reagents and ultrapure water generated from an ELGA laboratory water purification system for all experiments – minimising the risk of introducing contaminants that might affect their results.
This study demonstrates a potential way to enhance the activity of natural grapevine–cane extract by applying oxidative coupling directly to an extract with an enriched stilbene content.
The results show that the processed extract has improved antimicrobial activity, strongly inhibiting the growth of both downy mildew and grey mould. This suggests that the approach could offer a way to maximise the effectiveness of treatment – potentially reducing the amount and/or number of doses that are necessary to prevent or treat these major grapevine diseases.
The next steps will be to carry out greenhouse and field testing to confirm whether the processed extract offers an effective solution against these common grape pathogens.
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Dr Alison Halliday
After completing an undergraduate degree in Biochemistry & Genetics at Sheffield University, Alison was awarded a PhD in Human Molecular Genetics at the University of Newcastle. She carried out five years as a Senior Postdoctoral Research Fellow at UCL, investigating the genes involved in childhood obesity syndrome. Moving into science communications, she spent ten years at Cancer Research UK engaging the public about the charity’s work. She now specialises in writing about research across the life sciences, medicine and health.