To achieve success in mammalian cell culture it is necessary to use extremely pure media and buffers. The use of Type I ultrapure water to effectively eliminate the presence of biologically active species and other contaminants which could interfere with the culturing is essential.
Ion chromatography (IC) is a form of liquid chromatography that is used to separate atomic or molecular ions for qualitative or quantitative analysis. As scientists demand ever more rapid analyses with smaller sample volumes, ultrapure water plays a key role in maintaining the sensitivity and reproducibility of IC applications.
Northern, Sothern and Western blotting techniques depend on the use of Type I ultrapure water to obtain high quality results.
The importance of the quality of the water used to prepare samples, standards and reagents for ultraviolet and visible (UV-VIS) spectophotometric techniques should not be underestimated.
ELGA® LabWater has worked with Metrohm to develop two water purification systems, the PURELAB® flex 5 and 6, to overcome the current limitations.
Polymerase Chain Reaction (PCR) techniques are in widespread use through the life science sector and it is vital that the water used is ultrapure and free from waterborne contaminants which may affect the test results.
Liquid chromatography mass spectrometry (LC-MS) is a powerful analytical technique and ultrapure water should be used to ensure good chromatographic performance and high quality mass spectral data.
HPLC and UHPLC are powerful analytical techniques which require ultrapure water to ensure that chromatographic performance is not affected.
GC-MS is the method of choice for the analysis of organic compounds that are volatile or semi-volatile in water. As the assays are extremely sensitive, they require the highest purity laboratory water in order to product high quality mass spectral data.
High purity is an essential requirement for ICP-OES, ensuring that any compounds which could potentially interfere with the determination of the elements under investigation are eliminated.