General Lab Water Requirement

Water is used across the lab in a plethora of different techniques and applications. From glassware washing to autoclave filling, water is a common reagent.
General Lab Water Requirement

 

Glassware washing

This is a routine practice where glassware and instruments can be washed either by hand or using specifically designed equipment. Type III water is sufficient for most applications, however for more sensitive techniques Type II should be used. For the critical applications such as cell culture, ICP-MS or clinical methods, glassware should be washed with ultrapure Type I water. 

 

Heating Baths

Heating baths are found in many types of labs. Often, it is thought that tap water will be sufficient for heating baths as the purpose is to keep constant and regulated temperatures. While this is true scaling poses a large problem and will affect the maintenance and product life. If the water is too pure on the other hand, rusting can occur. Type III water is, therefore, sufficient for heating baths.

 

Autoclave Filling

Autoclaves are used in medical applications for the sterilization of equipment. This is done by subjecting the items to high-pressure steam to get the temperatures of the water greater than 100oC. 

 

Steam Generators

Steam generators are used in a range of applications, including clean room humidification, autoclaves and sterilisers. Most steam generators would benefit from pre-treatment of the water to avoid build-up. Type III water can be used for steam generators, however, if the steam is being used in disinfection services in healthcare environments, it is advised to check the strict specifications which are applied by some authorities.
 

Impact of water

Water is important in so many areas of the laboratory. While we typically focus on the technique itself, the water that is used in general lab processes, such as glassware washing, are just as important as residue from the water could impact on critical applications through contamination. 

Most of the cases in general lab requirements need to take into consideration the effect of scaling by using simple tap water. Impurities in the water will impact on the efficiency, maintenance and life of the products. Type III water helps to mitigate these issues.

Water Requirements

Make sure that you are using the right water type for your application. Here are the requirements for general lab applications.

  Sensitivity required Resistivity 
(MΩ.cm)*
TOC 
(ppb)
Filter 
(µm)
Bacteria (CFU/ml) Endotoxins (EU/ml) Nucleases Water grade
Glassware washing

General

High

>1

>18

<50

<10

<0.2

<0.2

<10

<1

NA

NA

NA

NA

II+

I

Steam generation

General

>1

<50

<0.2

<1

NA

NA

I

Feed to stills Low > 0.05 <500 NA NA NA NA Primary
Feed to ultra pure water systems General High

> 0.05

>1

< 50

< 10

NA

< 0.2

NA

<1

NA

NA

NA

NA

Primary

Ultra pure

Sample dilution and reagent preparation General High

>1

>18

< 50

< 10

< 0.2

< 0.2

<1

<1

NA

NA

NA

NA

General lab

Ultra pure

Solid phase extraction General High

>1

>18

< 50

< 3

< 0.2

< 0.2

<10

<1

NA

NA

NA

NA

General lab

Ultra pure

Steam generation General >1 < 50 < 0.2 <1 NA NA General lab
Water analysis General High

>5

>18

< 50

< 10

< 0.2

< 0.2

<10

<1

NA

NA

NA

NA

General lab

Ultra pure

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